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THG1L Polyclonal antibody

THG1L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15208-1-AP

Synonyms

ICF45, IHG-1, THG1L

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using THG1L Polyclonal antibody (15208-1-AP)

    WB analysis of K-562 using 15208-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15208-1-AP (THG1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using THG1L Polyclonal antibody (15208-1-AP)

    IHC staining of human endometrial cancer using 15208-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15208-1-AP (THG1L antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15208-1-AP targets THG1L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THG1L fusion protein Ag7361
Full Name tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001852
Gene Symbol THG1L
Gene ID (NCBI) 54974
RRIDAB_2303248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THG1L antibody 15208-1-APDownload protocol
IHC protocol for THG1L antibody 15208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Biochim Biophys Acta

Profibrotic IHG-1 complexes with renal disease associated HSPA5 and TRAP1 in mitochondria.

Authors - Una Bhreathnach
View Article