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  • Featured Product
  • KD/KO Validated

THOC6 Polyclonal antibody

THOC6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15316-1-AP

Synonyms

fSAP35, THO complex subunit 6 homolog, THOC6, WDR58

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOC6 Polyclonal antibody (15316-1-AP)

    WB analysis using 15316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using THOC6 Polyclonal antibody (15316-1-AP)

    WB analysis of mouse ovary using 15316-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using THOC6 Polyclonal antibody (15316-1-AP)

    IP experiment of A431 using 15316-1-AP

    IP result of anti-THOC6 (IP:15316-1-AP, 4ug; Detection:15316-1-AP 1:300) with A431 cells lysate 2280 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using THOC6 Polyclonal antibody (15316-1-AP)

    IHC staining of human ovary tumor using 15316-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15316-1-AP (THOC6 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, mouse ovary tissue, Neuro-2a cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15316-1-AP targets THOC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC6 fusion protein Ag7561
Full Name THO complex 6 homolog (Drosophila)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC003118
Gene Symbol THOC6
Gene ID (NCBI) 79228
RRIDAB_2240482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THOC6 antibody 15316-1-APDownload protocol
IHC protocol for THOC6 antibody 15316-1-APDownload protocol
IP protocol for THOC6 antibody 15316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article