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THRAP3 Polyclonal antibody

THRAP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19744-1-AP

Synonyms

THRAP3, TRAP150

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THRAP3 Polyclonal antibody (19744-1-AP)

    WB analysis using 19744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using THRAP3 Polyclonal antibody (19744-1-AP)

    WB analysis using 19744-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THRAP3 Polyclonal antibody (19744-1-AP)

    WB analysis of HeLa using 19744-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using THRAP3 Polyclonal antibody (19744-1-AP)

    IP experiment of HeLa using 19744-1-AP

    IP result of anti-THRAP3 (IP:19744-1-AP, 4ug; Detection:19744-1-AP 1:500) with HeLa cells lysate 600ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using THRAP3 Polyclonal antibody (19744-1-AP)

    IHC staining of human ovary tumor using 19744-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using THRAP3 Polyclonal antibody (19744-1-AP)

    IHC staining of human ovary tumor using 19744-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using THRAP3 Polyclonal antibody (19744-1-AP)

    IHC staining of human thyroid cancer using 19744-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19744-1-AP (THRAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THRAP3 Polyclonal antibody (19744-1-AP)

    IF Staining of HepG2 using 19744-1-AP

    Immunofluorescent analysis of HepG2 cells using 19744-1-AP (THRAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19744-1-AP targets THRAP3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name thyroid hormone receptor associated protein 3
Calculated Molecular Weight 109 kDa
Observed Molecular Weight 109-130 kDa
GenBank Accession NumberNM_005119
Gene Symbol THRAP3
Gene ID (NCBI) 9967
RRIDAB_11232035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3.

Protocols

Product Specific Protocols
WB protocol for THRAP3 antibody 19744-1-APDownload protocol
IHC protocol for THRAP3 antibody 19744-1-APDownload protocol
IF protocol for THRAP3 antibody 19744-1-APDownload protocol
IP protocol for THRAP3 antibody 19744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
View Article
IF

J Cell Sci

Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.

Authors - Valentina Iadevaia
View Article
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
View Article
humanWB,CoIP

Mol Cell Proteomics

Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics

Authors - Li Gao
View Article