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THUMPD3 Polyclonal antibody

THUMPD3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19807-1-AP

Synonyms

THUMP domain containing 3, THUMPD3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of HeLa using 19807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of mouse kidney using 19807-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of HepG2 using 19807-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human testis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human testis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    IF Staining of HeLa using 19807-1-AP

    Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    IF Staining of HepG2 using 19807-1-AP

    Immunofluorescent analysis of HepG2 cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19807-1-AP is THUMPD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THUMPD3 fusion protein Ag13833
Full Name THUMP domain containing 3
Calculated Molecular Weight 507 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC001622
Gene Symbol THUMPD3
Gene ID (NCBI) 25917
RRIDAB_10644140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THUMPD3 antibody 19807-1-APDownload protocol
IHC protocol for THUMPD3 antibody 19807-1-APDownload protocol
IF protocol for THUMPD3 antibody 19807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols