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THYN1 Polyclonal antibody

THYN1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15867-1-AP

Synonyms

HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THYN1 Polyclonal antibody (15867-1-AP)

    WB analysis using 15867-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using THYN1 Polyclonal antibody (15867-1-AP)

    WB analysis of HEK-293 using 15867-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using THYN1 Polyclonal antibody (15867-1-AP)

    WB analysis of mouse lung using 15867-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THYN1 Polyclonal antibody (15867-1-AP)

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THYN1 Polyclonal antibody (15867-1-AP)

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THYN1 Polyclonal antibody (15867-1-AP)

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THYN1 Polyclonal antibody (15867-1-AP)

    IHC staining of human lung cancer using 15867-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THYN1 Polyclonal antibody (15867-1-AP)

    IF Staining of HepG2 using 15867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15867-1-AP (THYN1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15867-1-AP targets THYN1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THYN1 fusion protein Ag8481
Full Name thymocyte nuclear protein 1
Calculated Molecular Weight225aa,26 kDa; 166aa,19 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC006978
Gene Symbol THYN1
Gene ID (NCBI) 29087
RRIDAB_2287179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)

Protocols

Product Specific Protocols
WB protocol for THYN1 antibody 15867-1-APDownload protocol
IHC protocol for THYN1 antibody 15867-1-APDownload protocol
IF protocol for THYN1 antibody 15867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols