TIAR Monoclonal antibody

TIAR Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8D12

Cat no : 66907-1-Ig

Synonyms

Nucleolysin TIAR, TCBP, TIA 1 related protein, TIAL1, TIAR



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HSC-T6 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66907-1-Ig targets TIAR in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIAR fusion protein Ag11981
Full Name TIA1 cytotoxic granule-associated RNA binding protein-like 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 39 and 41 kDa
GenBank Accession NumberBC030025
Gene Symbol TIAL1
Gene ID (NCBI) 7073
RRIDAB_2882234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

Protocols

Product Specific Protocols
WB protocol for TIAR antibody 66907-1-IgDownload protocol
IHC protocol for TIAR antibody 66907-1-IgDownload protocol
IF protocol for TIAR antibody 66907-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biosci

Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.

Authors - Yuzo Abe
humanWB

J Cell Mol Med

LOXL1-AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR-374b-5p/MMP14 axis.

Authors - Bolong Yi
humanIF

Research (Wash D C)

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

Authors - Fengli Wang
humanIF

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang