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  • KD/KO Validated

TIMELESS Polyclonal antibody

TIMELESS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14421-1-AP

Synonyms

hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IHC detected inhuman spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14421-1-AP targets TIMELESS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMELESS fusion protein Ag5767
Full Name timeless homolog (Drosophila)
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC050557
Gene Symbol TIMELESS
Gene ID (NCBI) 8914
RRIDAB_2201962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Protocols

Product Specific Protocols
WB protocol for TIMELESS antibody 14421-1-APDownload protocol
IHC protocol for TIMELESS antibody 14421-1-APDownload protocol
IF protocol for TIMELESS antibody 14421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Lett

TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.

Authors - Sai-Lan Liu
  • KD Validated
humanWB

Cell Death Dis

MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS.

Authors - Fangfang Li
  • KD Validated
humanWB

Cell Prolif

Circadian clock genes promote glioma progression by affecting tumour immune infiltration and tumour cell proliferation.

Authors - Zeyu Wang
humanWB,IHC

Gut Microbes

Exosomal-miR-129-2-3p derived from Fusobacterium nucleatum-infected intestinal epithelial cells promotes experimental colitis through regulating TIMELESS-mediated cellular senescence pathway

Authors - Shuchun Wei
  • KD Validated
humanWB

Cell Signal

HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-β-catenin pathway

Authors - Zhaoxia Wang