• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TIN2 Polyclonal antibody

TIN2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, chip, ELISA

Conjugate

Unconjugated

Cat no : 11368-1-AP

Synonyms

TIN2, TIN2L, TINF2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse spleen tissue using TIN2 Polyclonal antibody (11368-1-AP)

    WB analysis of mouse spleen using 11368-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11368-1-AP (TIN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TIN2 Polyclonal antibody (11368-1-AP)

    IF Staining of HepG2 using 11368-1-AP

    Immunofluorescent analysis of HepG2 cells, using TINF2 antibody 11368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11368-1-AP targets TIN2 in WB, IF, chip, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIN2 fusion protein Ag1922
Full Name TERF1 (TRF1)-interacting nuclear factor 2
Calculated Molecular Weight 451 aa, 50 kDa
Observed Molecular Weight 50 kDa, 40 kDa
GenBank Accession NumberBC005030
Gene Symbol TINF2
Gene ID (NCBI) 26277
RRIDAB_2205100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIN2, also named as TINF2, is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TIN2 has some isoforms with MW 50 kDa (TIN2L), 40 kDa (TIN2S) and 13 kDa. This antibody detects TIN2L, TIN2S and the shelterin complex.

Protocols

Product Specific Protocols
WB protocol for TIN2 antibody 11368-1-APDownload protocol
IF protocol for TIN2 antibody 11368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchip

Oncotarget

BRCA1/2 mutations perturb telomere biology: characterization of structural and functional abnormalities in vitro and in vivo.

Authors - Orit Uziel
View Article
human

Cells

The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells.

Authors - Tomer Harpaz
View Article
humanWB

Sci Adv

Structural basis of telomeric nucleosome recognition by shelterin factor TRF1

Authors - Hongmiao Hu
View Article