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TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2H11

Cat no : 66216-1-Ig

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66216-1-Ig targets TLE1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLE1 fusion protein Ag21327
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
RRIDAB_2881607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.

Protocols

Product Specific Protocols
WB protocol for TLE1 antibody 66216-1-IgDownload protocol
IHC protocol for TLE1 antibody 66216-1-IgDownload protocol
FC protocol for TLE1 antibody 66216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols