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TLE1 Monoclonal antibody
TLE1 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2F2H11
Cat no : 66216-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66216-1-Ig targets TLE1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLE1 fusion protein Ag21327 |
Full Name | transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) |
Calculated Molecular Weight | 83 kDa |
Observed Molecular Weight | 85-95 kDa |
GenBank Accession Number | BC010100 |
Gene Symbol | TLE1 |
Gene ID (NCBI) | 7088 |
RRID | AB_2881607 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.
Protocols
Product Specific Protocols | |
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WB protocol for TLE1 antibody 66216-1-Ig | Download protocol |
IHC protocol for TLE1 antibody 66216-1-Ig | Download protocol |
FC protocol for TLE1 antibody 66216-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |