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TLE3 Monoclonal antibody

TLE3 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A8

Cat no : 66083-1-Ig

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using human TLE3 Monoclonal antibody (66083-1-Ig)

    WB analysis of HEK-293 using 66083-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using human TLE3 Monoclonal antibody (66083-1-Ig)

    WB analysis of human brain using 66083-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TLE3 Monoclonal antibody (66083-1-Ig)

    IP experiment of HEK-293 using 66083-1-Ig

    IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Monoclonal antibody (66083-1-Ig)

    IHC staining of human breast cancer using 66083-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TLE3 Monoclonal antibody (66083-1-Ig)

    IHC staining of human breast cancer using 66083-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66083-1-Ig targets TLE3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17906
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 772 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
RRIDAB_11182267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 66083-1-IgDownload protocol
IHC protocol for TLE3 antibody 66083-1-IgDownload protocol
IP protocol for TLE3 antibody 66083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols