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TM7SF2 Polyclonal antibody

TM7SF2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12033-1-AP

Synonyms

Another new gene 1 protein, C 14 sterol reductase, Delta 14 SR, Delta(14) sterol reductase, DHCR14A, NET47, Putative sterol reductase SR 1, Sterol C14 reductase, TM7SF2

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using TM7SF2 Polyclonal antibody (12033-1-AP)

    WB analysis of human lung using 12033-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TM7SF2 Polyclonal antibody (12033-1-AP)

    WB analysis of human kidney using 12033-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TM7SF2 Polyclonal antibody (12033-1-AP)

    WB analysis of human heart using 12033-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TM7SF2 Polyclonal antibody (12033-1-AP)

    WB analysis of PC-3 using 12033-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TM7SF2 Polyclonal antibody (12033-1-AP)

    WB analysis of BxPC-3 using 12033-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TM7SF2 Polyclonal antibody (12033-1-AP)

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TM7SF2 Polyclonal antibody (12033-1-AP)

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TM7SF2 Polyclonal antibody (12033-1-AP)

    IF Staining of PC-3 using 12033-1-AP

    Immunofluorescent analysis of PC-3 cells using 12033-1-AP (TM7SF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman lung tissue, BxPC-3 cells, human heart tissue, human kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12033-1-AP targets TM7SF2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TM7SF2 fusion protein Ag2637
Full Name transmembrane 7 superfamily member 2
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC012857
Gene Symbol TM7SF2
Gene ID (NCBI) 7108
RRIDAB_2203934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TM7SF2 antibody 12033-1-APDownload protocol
IHC protocol for TM7SF2 antibody 12033-1-APDownload protocol
IF protocol for TM7SF2 antibody 12033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.

Authors - Monika Lewinska
View Article