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TMC1 Polyclonal antibody

TMC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20718-1-AP

Synonyms

DFNA36, DFNB11, DFNB7, TMC1, transmembrane channel like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TMC1 Polyclonal antibody (20718-1-AP)

    WB analysis of mouse brain using 20718-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20718-1-AP (TMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TMC1 Polyclonal antibody (20718-1-AP)

    IHC staining of human placenta using 20718-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20718-1-AP (TMC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TMC1 Polyclonal antibody (20718-1-AP)

    IHC staining of human placenta using 20718-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20718-1-AP (TMC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TMC1 Polyclonal antibody (20718-1-AP)

    IF Staining of mouse brain using 20718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TMC1 antibody (20718-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TMC1 Polyclonal antibody (20718-1-AP)

    IF Staining of mouse brain using 20718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TMC1 antibody (20718-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20718-1-AP targets TMC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transmembrane channel-like 1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberNM_138691
Gene Symbol TMC1
Gene ID (NCBI) 117531
RRIDAB_10693676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane channel-like protein 1 (TMC1), is an integral membrane protein with six transmembrane domains and cytoplasmically oriented N- and C- termini (PMID: 20672865). Human TMC1 and mouse Tmc1 mRNA expression were detected in human fetal cochlea and inner-ear tissue of postnatal mouse, respectively. TMC1 is required for cochlear hair-cell function (PMID: 11850618). It is a component of hair cell transduction channels and contributes to permeation properties (PMID: 23871232). Mutations in TMC1 gene can cause progressive postlingual hearing loss and profound prelingual deafness (PMID: 11850618). The antibody is specific to TMC1.

Protocols

Product Specific Protocols
WB protocol for TMC1 antibody 20718-1-APDownload protocol
IHC protocol for TMC1 antibody 20718-1-APDownload protocol
IF protocol for TMC1 antibody 20718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols