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TMEM27 Polyclonal antibody

TMEM27 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19207-1-AP

Synonyms

ACE2, CLTRN, Collectrin, NX 17, NX17, TMEM27, transmembrane protein 27

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    WB analysis of mouse pancreas using 19207-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    WB analysis of mouse kidney using 19207-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    WB analysis of mouse kidney using 19207-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    IHC staining of human kidney using 19207-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    IHC staining of human kidney using 19207-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    IHC staining of human liver cancer using 19207-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19207-1-AP (TMEM27 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM27 Polyclonal antibody (19207-1-AP)

    IHC staining of human liver cancer using 19207-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19207-1-AP (TMEM27 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM27 Polyclonal antibody (19207-1-AP)

    IF Staining of HepG2 using 19207-1-AP

    Immunofluorescent analysis of HepG2 cells, using TMEM27 antibody 19207-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse pancreas tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19207-1-AP targets TMEM27 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM27 fusion protein Ag7668
Full Name transmembrane protein 27
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC050606
Gene Symbol TMEM27
Gene ID (NCBI) 57393
RRIDAB_10860104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).

Protocols

Product Specific Protocols
WB protocol for TMEM27 antibody 19207-1-APDownload protocol
IHC protocol for TMEM27 antibody 19207-1-APDownload protocol
IF protocol for TMEM27 antibody 19207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols