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TMEM27 Polyclonal antibody

TMEM27 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 24059-1-AP

Synonyms

Collectrin, NX 17, NX17, TMEM27, transmembrane protein 27

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TMEM27 Polyclonal antibody (24059-1-AP)

    WB analysis of HEK-293 using 24059-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24059-1-AP (TMEM27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24059-1-AP is TMEM27 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM27 fusion protein Ag21257
Full Name transmembrane protein 27
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC050606
Gene Symbol TMEM27
Gene ID (NCBI) 57393
RRIDAB_2879419
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).

Protocols

Product Specific Protocols
WB protocol for TMEM27 antibody 24059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols