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  • Featured Product
  • KD/KO Validated

TMEM38B Polyclonal antibody

TMEM38B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19919-1-AP

Synonyms

C9orf87, D4Ertd89e, TMEM38B, transmembrane protein 38B, TRIC B, TRICB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis using 19919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19919-1-AP (TMEM38B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis of MCF-7 using 19919-1-AP

    WB result of TMEM38B antibody (19919-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM38B transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM38B Polyclonal antibody (19919-1-AP)

    IF Staining of HeLa using 19919-1-AP

    Immunofluorescent analysis of HeLa cells using 19919-1-AP (TMEM38B antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19919-1-AP targets TMEM38B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM38B fusion protein Ag13835
Full Name transmembrane protein 38B
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC000049
Gene Symbol TMEM38B
Gene ID (NCBI) 55151
RRIDAB_2878623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM38B, also named as Trimeric intracellular cation channel type B, is a 291 amino acid protein, which belongs to the TMEM38 family. TMEM38B localizes in the endoplasmic reticulum and may act as a potassium counter-ion channel that functions in synchronization with calcium release from intracellular stores. TMEM38B has an association with Osteogenesis imperfecta 14.

Protocols

Product Specific Protocols
WB protocol for TMEM38B antibody 19919-1-APDownload protocol
IHC protocol for TMEM38B antibody 19919-1-APDownload protocol
IF protocol for TMEM38B antibody 19919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols