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TMPRSS6 Polyclonal antibody

TMPRSS6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 12950-1-AP

Synonyms

IRIDA, Matriptase 2, TMPRSS6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using TMPRSS6 Polyclonal antibody (12950-1-AP)

    WB analysis of HEK-293 using 12950-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMPRSS6 Polyclonal antibody (12950-1-AP)

    WB analysis of MCF-7 using 12950-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TMPRSS6 Polyclonal antibody (12950-1-AP)

    WB analysis of HepG2 using 12950-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TMPRSS6 Polyclonal antibody (12950-1-AP)

    WB analysis of mouse liver using 12950-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12950-1-AP targets TMPRSS6 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMPRSS6 fusion protein Ag4076
Full Name transmembrane protease, serine 6
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC039082
Gene Symbol TMPRSS6
Gene ID (NCBI) 164656
RRIDAB_2877896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The type II transmembrane serine protease TMPRSS6 (also known as matriptase-2) controls iron homeostasis through its negative regulation of expression of hepcidin, a key hormone involved in iron metabolism. Upstream of the hepcidin-regulated signaling pathway, TMPRSS6 cleaves its target substrate hemojuvelin (HJV) at the plasma membrane(PMID:21724843).It Belongs to the peptidase S1 family.Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA).It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TMPRSS6 antibody 12950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Haematologica

New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia.

Authors - Jing Liu
View Article