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TNFSF18 Polyclonal antibody

TNFSF18 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

Cat no : 23899-1-AP

Synonyms

AITRL, GITRL, hGITRL, TL6, TNFSF18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFSF18 Polyclonal antibody (23899-1-AP)

    WB analysis using 23899-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 23899-1-AP (TNFSF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of human thyroid cancer using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of human thyroid cancer using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of mouse testis using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of mouse testis using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of human thyroid cancer using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFSF18 Polyclonal antibody (23899-1-AP)

    IHC staining of human thyroid cancer using 23899-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23899-1-AP is TNFSF18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFSF18 fusion protein Ag20979
Full Name tumor necrosis factor (ligand) superfamily, member 18
Calculated Molecular Weight 177 aa, 20 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC069319
Gene Symbol TNFSF18
Gene ID (NCBI) 8995
RRIDAB_2879355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNFSF18 antibody 23899-1-APDownload protocol
IHC protocol for TNFSF18 antibody 23899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF,FC

Cell Death Dis

GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt.

Authors - Yu He
View Article
mouseIF

Cell Death Dis

GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice

Authors - Li Li
View Article