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  • Featured Product
  • KD/KO Validated

TNPO2 Polyclonal antibody

TNPO2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17831-1-AP

Synonyms

IPO3, Karyopherin beta 2b, KPNB2B, TNPO2, transportin 2, TRN2

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using TNPO2 Polyclonal antibody (17831-1-AP)

    WB analysis of fetal human brain using 17831-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TNPO2 Polyclonal antibody (17831-1-AP)

    WB analysis of HeLa using 17831-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TNPO2 Polyclonal antibody (17831-1-AP)

    WB analysis of Jurkat using 17831-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TNPO2 Polyclonal antibody (17831-1-AP)

    WB analysis of HEK-293 using 17831-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TNPO2 Polyclonal antibody (17831-1-AP)

    WB analysis of K-562 using 17831-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TNPO2 Polyclonal antibody (17831-1-AP)

    IF Staining of MCF-7 using 17831-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TNPO2 antibody 17831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected infetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17831-1-AP targets TNPO2 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNPO2 fusion protein Ag12229
Full Name transportin 2
Calculated Molecular Weight 887 aa, 100 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC072420
Gene Symbol TNPO2
Gene ID (NCBI) 30000
RRIDAB_10598481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNPO2 antibody 17831-1-APDownload protocol
IF protocol for TNPO2 antibody 17831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Authors - Eva-Maria Hock
View Article
humanWB,IF

Sci Rep

Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.

Authors - Tetsuji Moriyama
  • KD Validated
View Article
mouseWB

eLife

Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling

Authors - Woong Y Hwang
View Article
humanWB,CoIP

Int J Biol Macromol

Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility

Authors - Yang Liu
View Article