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TOB2 Polyclonal antibody

TOB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13607-1-AP

Synonyms

KIAA1663, Protein Tob2, Protein Tob4, TOB2, TOB4, TOBL, Transducer of erbB 2 2, transducer of ERBB2, 2, TROB2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TOB2 Polyclonal antibody (13607-1-AP)

    WB analysis using 13607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13607-1-AP (TOB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TOB2 Polyclonal antibody (13607-1-AP)

    IHC staining of human kidney using 13607-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13607-1-AP (TOB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TOB2 Polyclonal antibody (13607-1-AP)

    IHC staining of human kidney using 13607-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13607-1-AP (TOB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOB2 Polyclonal antibody (13607-1-AP)

    IF Staining of HepG2 using 13607-1-AP

    Immunofluorescent analysis of HepG2 cells using 13607-1-AP (TOB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13607-1-AP targets TOB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOB2 fusion protein Ag4491
Full Name transducer of ERBB2, 2
Calculated Molecular Weight 344 aa, 37 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC038957
Gene Symbol TOB2
Gene ID (NCBI) 10766
RRIDAB_2877961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TOB2 antibody 13607-1-APDownload protocol
IHC protocol for TOB2 antibody 13607-1-APDownload protocol
IF protocol for TOB2 antibody 13607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

bioRxiv

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article