• Featured Product
  • KD/KO Validated

TOE1 Polyclonal antibody

TOE1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16203-1-AP

Synonyms

Target of EGR1 protein 1, TOE1



Tested Applications

Positive WB detected inHEK-293 cells, mouse thymus tissue, HeLa cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

16203-1-AP targets TOE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOE1 fusion protein Ag9172
Full Name target of EGR1, member 1 (nuclear)
Calculated Molecular Weight 510 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC009364
Gene Symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_2207278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
WB protocol for TOE1 antibody 16203-1-APDownload protocol
IHC protocol for TOE1 antibody 16203-1-APDownload protocol
IF protocol for TOE1 antibody 16203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Theriogenology

Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice

Authors - Hongcheng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rhys (Verified Customer) (08-26-2019)

Superb antibody that works well for the detection for endogenous human TOE1 protein by western blotting.Lane 1: K562 wtGapLane 2: K562 control (empty vector)Lane 3: K562 TOE1 overexpressionGapLane 4: K562 scrambled shRNA controlLane 5: K562 TOE1 shRNA #1Lane 6: K562 TOE1 shRNA #2Lane 7: K562 TOE1 shRNA #3Lane 8: K562 TOE1 shRNA #4Lane 9: K562 TOE1 shRNA #5

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: K562 cells (Chronic Myeloid Leukaemia)
TOE1 Antibody Western Blot, validation (1/1000 dilution) in K562 cells (Chronic Myeloid Leukaemia) (Cat no:16203-1-AP)