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TOMM6 Polyclonal antibody

TOMM6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16689-1-AP

Synonyms

OBTP, TOM6, TOMM6

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using TOMM6 Polyclonal antibody (16689-1-AP)

    WB analysis of human skeletal muscle using 16689-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16689-1-AP (TOMM6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TOMM6 Polyclonal antibody (16689-1-AP)

    WB analysis of MCF-7 using 16689-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16689-1-AP (TOMM6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TOMM6 Polyclonal antibody (16689-1-AP)

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TOMM6 Polyclonal antibody (16689-1-AP)

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16689-1-AP targets TOMM6 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOMM6 fusion protein Ag10037
Full Name translocase of outer mitochondrial membrane 6 homolog (yeast)
Calculated Molecular Weight 74 aa, 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC018624
Gene Symbol TOMM6
Gene ID (NCBI) 100188893
RRIDAB_2240919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOMM6, also named as OBTP and TOM6, is a part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

Protocols

Product Specific Protocols
WB protocol for TOMM6 antibody 16689-1-APDownload protocol
IHC protocol for TOMM6 antibody 16689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols