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TOP2A Monoclonal antibody
TOP2A Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E12B3
Cat no : 66541-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, L02 cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
Product Information
66541-1-Ig targets TOP2A in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TOP2A fusion protein Ag19551 |
| Full Name | topoisomerase (DNA) II alpha 170kDa |
| Calculated Molecular Weight | 1531 aa, 174 kDa |
| Observed Molecular Weight | 174 kDa |
| GenBank Accession Number | BC140791 |
| Gene Symbol | TOP2A |
| Gene ID (NCBI) | 7153 |
| RRID | AB_2881903 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOP2A antibody 66541-1-Ig | Download protocol |
| IHC protocol for TOP2A antibody 66541-1-Ig | Download protocol |
| IF protocol for TOP2A antibody 66541-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Identification of differentially expressed genes in non-small cell lung cancer. | ||
Front Oncol TOP2A correlates with poor prognosis and affects radioresistance of medulloblastoma
| ||
Reprod Biol Endocrinol TOP2A deficit-induced abnormal decidualization leads to recurrent implantation failure via the NF-κB signaling pathway
|
