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- KD/KO Validated
TOP3A Polyclonal antibody
TOP3A Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14525-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, HL-60 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 23 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
14525-1-AP targets TOP3A in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOP3A fusion protein Ag6010 |
| Full Name | topoisomerase (DNA) III alpha |
| Calculated Molecular Weight | 1001 aa, 112 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC051748 |
| Gene Symbol | TOP3A |
| Gene ID (NCBI) | 7156 |
| RRID | AB_2205881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOP3A antibody 14525-1-AP | Download protocol |
| IP protocol for TOP3A antibody 14525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation. | ||
Mol Cell Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
| ||
Sci Adv The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication. | ||
Nat Commun The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. | ||
Nucleic Acids Res Top3α is the replicative topoisomerase in mitochondrial DNA replication.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andrea (Verified Customer) (01-23-2023) | Strong and crisp band.
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