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Torsin A Polyclonal antibody

Torsin A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10296-1-AP

Synonyms

DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Torsin A Polyclonal antibody (10296-1-AP)

    WB analysis of mouse brain using 10296-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Torsin A Polyclonal antibody (10296-1-AP)

    WB analysis of rat brain using 10296-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Torsin A Polyclonal antibody (10296-1-AP)

    WB analysis of mouse liver using 10296-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Torsin A Polyclonal antibody (10296-1-AP)

    WB analysis of mouse kidney using 10296-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Torsin A Polyclonal antibody (10296-1-AP)

    WB analysis of HEK-293 using 10296-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Torsin A Polyclonal antibody (10296-1-AP)

    IHC staining of human colon cancer using 10296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10296-1-AP (Torsin A antibody) at dilution of 1:100 (under 25x lens).

Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, mouse kidney tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10296-1-AP targets Torsin A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Torsin A fusion protein Ag0358
Full Name torsin family 1, member A (torsin A)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC000674
Gene Symbol TOR1A
Gene ID (NCBI) 1861
RRIDAB_2206116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.

Protocols

Product Specific Protocols
WB protocol for Torsin A antibody 10296-1-APDownload protocol
IHC protocol for Torsin A antibody 10296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurobiol Dis

Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease

Authors - Ened Rodríguez-Urgellés
View Article