PIG3 Polyclonal antibody

PIG3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14828-1-AP

Synonyms

p53 induced gene 3 protein, PIG3, Quinone oxidoreductase PIG3, TP53I3



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14828-1-AP targets PIG3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIG3 fusion protein Ag6618
Full Name tumor protein p53 inducible protein 3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC000474
Gene Symbol TP53I3
Gene ID (NCBI) 9540
RRIDAB_2206893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIG3 antibody 14828-1-APDownload protocol
IHC protocol for PIG3 antibody 14828-1-APDownload protocol
IF protocol for PIG3 antibody 14828-1-APDownload protocol
IP protocol for PIG3 antibody 14828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biomaterials

Furin-instructed aggregated gold nanoparticles for re-educating tumor associated macrophages and overcoming breast cancer chemoresistance.

Authors - Rou Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

IGNACIO (Verified Customer) (10-22-2021)

The antibody works perfect for WB with strong bands. The size is correct. Highly recommended.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: U2OS cancer cell lines