• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TPH2 Polyclonal antibody

TPH2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22590-1-AP

Synonyms

NTPH, TPH2, Tryptophan 5 hydroxylase 2, Tryptophan 5 monooxygenase 2, tryptophan hydroxylase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat brain tissue using TPH2 Polyclonal antibody (22590-1-AP)

    WB analysis of rat brain using 22590-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22590-1-AP (TPH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TPH2 Polyclonal antibody (22590-1-AP)

    IHC staining of human liver cancer using 22590-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22590-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TPH2 Polyclonal antibody (22590-1-AP)

    IHC staining of human liver cancer using 22590-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22590-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPH2 Polyclonal antibody (22590-1-AP)

    IHC staining of mouse brain using 22590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22590-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPH2 Polyclonal antibody (22590-1-AP)

    IHC staining of mouse brain using 22590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22590-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22590-1-AP is TPH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPH2 fusion protein Ag18408
Full Name tryptophan hydroxylase 2
Calculated Molecular Weight 490 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC114442
Gene Symbol TPH2
Gene ID (NCBI) 121278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Neurosci

PMID: 31371425

Authors - Norifumi Shioda
View Article
ratIF

Neurosci Bull

Genetic Architecture and Functional Implications of the CSF-Contacting Nucleus

Authors - Siyuan Song
View Article