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TPI1 Monoclonal antibody

TPI1 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

1G2E9

Cat no : 60036-1-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TPI1 Monoclonal antibody (60036-1-Ig)

    WB analysis of HeLa using 60036-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60036-1-Ig (TPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60036-1-Ig targets TPI1 in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen TPI1 fusion protein Ag1175
Full Name triosephosphate isomerase 1
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Protocols

Product Specific Protocols
WB protocol for TPI1 antibody 60036-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols