TPM1 Polyclonal antibody

TPM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 28477-1-AP

Synonyms

Alpha tropomyosin, C15orf13, CMD1Y, HTM alpha, TMSA, TPM1, Tropomyosin 1, tropomyosin 1 (alpha), Tropomyosin alpha 1 chain



Tested Applications

Positive WB detected inU-251 cells, mouse heart, mouse lung, rat lung
Positive IHC detected inhuman stomach cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28477-1-AP targets TPM1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPM1 fusion protein Ag29525
Full Name tropomyosin 1 (alpha)
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053545
Gene Symbol TPM1
Gene ID (NCBI) 7168
RRIDAB_2881151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TPM1 antibody 28477-1-APDownload protocol
IHC protocol for TPM1 antibody 28477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bone Res

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

Authors - Ming Luo
WB

Genes Dev

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

Authors - Megan L Norris
ratWB

Genomics

Comprehensive analysis of transcriptome-wide N6-methyladenosine methylomes in the Barrett's esophagus in rats

Authors - Ke Zou
mouse,rabbitWB,IF,CoIP

Adv Sci (Weinh)

A Circular RNA Generated from Nebulin (NEB) Gene Splicing Promotes Skeletal Muscle Myogenesis in Cattle as Detected by a Multi-Omics Approach

Authors - Kongwei Huang