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TPM4 Monoclonal antibody

TPM4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D12

Cat no : 67244-1-Ig

Synonyms

TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis using 67244-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TPM4 Monoclonal antibody (67244-1-Ig)

    WB analysis of A549 using 67244-1-Ig

    WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TPM4 Monoclonal antibody (67244-1-Ig)

    IHC staining of mouse heart using 67244-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using TPM4 Monoclonal antibody (67244-1-Ig)

    IHC staining of rat heart using 67244-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat skeletal muscle tissue, A549 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, rabbit skeletal muscle tissue, rat heart tissue, mouse heart tissue, rabbit heart tissue, HT-29 cells, human heart tissue, pig heart tissue
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67244-1-Ig targets TPM4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPM4 fusion protein Ag6947
Full Name tropomyosin 4
Calculated Molecular Weight 248 aa, 29 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC037576
Gene Symbol TPM4
Gene ID (NCBI) 7171
RRIDAB_2882523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.

Protocols

Product Specific Protocols
WB protocol for TPM4 antibody 67244-1-IgDownload protocol
IHC protocol for TPM4 antibody 67244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Rheumatology (Oxford)

Association of Anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis

Authors - Rie Karasawa
View Article