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TPSAB1 Polyclonal antibody

TPSAB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13343-1-AP

Synonyms

MCP7, TPS1, TPS2, TPSAB1, TPSB1, Tryptase 1, tryptase alpha/beta 1, Tryptase beta 1, Tryptase I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPSAB1 Polyclonal antibody (13343-1-AP)

    WB analysis using 13343-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    WB analysis of A431 using 13343-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IP experiment of mouse liver using 13343-1-AP

    IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendix tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human appendix using 13343-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IF Staining of mouse skin using 13343-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    IF Staining of HepG2 using 13343-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of U-937 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    FC experiment of U-937 using 13343-1-AP

    1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Tested Applications

Positive WB detected inA431 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman tonsillitis tissue, human appendix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skin tissue, HepG2 cells
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13343-1-AP targets TPSAB1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPSAB1 fusion protein Ag4167
Full Name tryptase alpha/beta 1
Calculated Molecular Weight 275 aa, 31 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC028059
Gene Symbol TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2206197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TPSAB1 antibody 13343-1-APDownload protocol
IHC protocol for TPSAB1 antibody 13343-1-APDownload protocol
IF protocol for TPSAB1 antibody 13343-1-APDownload protocol
IP protocol for TPSAB1 antibody 13343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Clin Transl Med

Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour

Authors - Kaiyuan Ji
View Article
ratIHC

Front Immunol

Palmatine treats urticaria by reducing inflammation and increasing autophagy

Authors - Tian Xiao
View Article
humanIHC

IUBMB Life

Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma.

Authors - Weijuan Lou
View Article
humanWB

BMC Cancer

The release of tryptase from mast cells promote tumor cell metastasis via exosomes.

Authors - Hui Xiao
View Article
mouseWB,IF

Int J Mol Sci

An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation

Authors - Jufang Jia
View Article
mouseIF

Ann Transl Med

Identification of hub genes and infiltrating immune cells in skeletal muscle in obesity

Authors - Shuoshuo Jin
View Article