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TPSAB1 Monoclonal antibody

TPSAB1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A10G1

Cat no : 66174-1-Ig

Synonyms

MCP7, TPS1, TPS2, TPSAB1, TPSB1, Tryptase 1, tryptase alpha/beta 1, Tryptase beta 1, Tryptase I

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    WB analysis of human kidney using 66174-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66174-1-Ig (TPSAB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    IHC staining of human tonsillitis using 66174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 10x lens) microwave oven heating mediated antigen retrieved with .

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    IHC staining of human tonsillitis using 66174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 40x lens) microwave oven heating mediated antigen retrieved with .

Tested Applications

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66174-1-Ig targets TPSAB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPSAB1 fusion protein Ag4167
Full Name tryptase alpha/beta 1
Calculated Molecular Weight 275 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC028059
Gene Symbol TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2881569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for TPSAB1 antibody 66174-1-IgDownload protocol
IHC protocol for TPSAB1 antibody 66174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncogene

Mast cells inhibit colorectal cancer development by inducing ER stress through secreting Cystatin C

Authors - Feifei Song
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsilitis tissue. HIER with citrate buffer

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
TPSAB1 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66174-1-Ig)