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TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat no : 66713-1-Ig

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of MCF-7 using 66713-1-Ig

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis using 66713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of HeLa using 66713-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of RAW 264.7 using 66713-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPT1 Monoclonal antibody (66713-1-Ig)

    IHC staining of human colon cancer using 66713-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPT1 Monoclonal antibody (66713-1-Ig)

    IHC staining of human colon cancer using 66713-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66713-1-Ig targets TPT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPT1 fusion protein Ag27651
Full Name tumor protein, translationally-controlled 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003352
Gene Symbol TPT1
Gene ID (NCBI) 7178
RRIDAB_2882064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

Protocols

Product Specific Protocols
WB protocol for TPT1 antibody 66713-1-IgDownload protocol
IHC protocol for TPT1 antibody 66713-1-IgDownload protocol
IF protocol for TPT1 antibody 66713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols