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CoraLite® Plus 488-conjugated TRAF4 Monoclonal antibody

TRAF4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C9A11

Cat no : CL488-66755

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TRAF4 Monoclonal ant (CL488-66755)

    IF Staining of HeLa using CL488-66755

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66755 (TRAF4 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66755 targets TRAF4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF4 fusion protein Ag27221
Full Name TNF receptor-associated factor 4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC001769
Gene Symbol TRAF4
Gene ID (NCBI) 9618
RRIDAB_2883380
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRAF4 antibody CL488-66755Download protocol
Standard Protocols
Click here to view our Standard Protocols