TRAK2 Polyclonal antibody

TRAK2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13770-1-AP

Synonyms

ALS2CR3, CALS C, GRIF 1, GRIF1, KIAA0549, OIP98, TRAK2



Tested Applications

Positive WB detected inhuman liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

13770-1-AP targets TRAK2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAK2 fusion protein Ag4753
Full Name trafficking protein, kinesin binding 2
Calculated Molecular Weight 914 aa, 101 kDa
Observed Molecular Weight 101-106 kDa
GenBank Accession NumberBC040668
Gene Symbol TRAK2
Gene ID (NCBI) 66008
RRIDAB_2207961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trafficking kinesin-binding protein 2 (TRAK2) is a member of the TRAK family of kinesin adaptor proteins. In mammals, there are two members of the TRAK family, TRAK1 and TRAK2 (PMID: 25653102). TRAK1 and TRAK2 play roles in lysosomal and mitochondrial trafficking in cells. They function as kinesin adaptors linking kinesin heavy chain (KHC) to mitochondria (PMID: 28364549). TRAK2 has been found to act as a trafficking factor for the K+ channel Kir2.1 and γ-Aminobutyric acid type A (GABAA) receptor (PMID: 16895905; 24122114).

Protocols

Product Specific Protocols
WB protocol for TRAK2 antibody 13770-1-APDownload protocol
IHC protocol for TRAK2 antibody 13770-1-APDownload protocol
IF protocol for TRAK2 antibody 13770-1-APDownload protocol
IP protocol for TRAK2 antibody 13770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Adaptation of mitochondrial network dynamics and velocity of mitochondrial movement to chronic stress present in fibroblasts derived from patients with sporadic form of Alzheimer's disease.

Authors - Karolina Drabik
ratWB

J Cell Sci

Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19.

Authors - Katarzyna Majstrowicz
mouseWB

Exp Cell Res

Antagomirs targeting miR-142-5p attenuate pilocarpine-induced status epilepticus in mice.

Authors - Haifeng Zhang
ratWB

Bioeng Transl Med

Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36

Authors - Zhijie Zhu