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TRAPPC3 Polyclonal antibody

TRAPPC3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15555-1-AP

Synonyms

BET3, BET3 homolog, TRAPPC3



Tested Applications

Positive WB detected inmouse liver tissue, PC-3 cells, HEK-293 cells, mouse small intestine tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

15555-1-AP targets TRAPPC3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC3 fusion protein Ag7924
Full Name trafficking protein particle complex 3
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC007662
Gene Symbol TRAPPC3
Gene ID (NCBI) 27095
RRIDAB_2208142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)

Protocols

Product Specific Protocols
WB protocol for TRAPPC3 antibody 15555-1-APDownload protocol
IHC protocol for TRAPPC3 antibody 15555-1-APDownload protocol
IF protocol for TRAPPC3 antibody 15555-1-APDownload protocol
IP protocol for TRAPPC3 antibody 15555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.

Authors - Bassik Michael C MC
humanWB

Proc Natl Acad Sci U S A

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.

Authors - Westlake Christopher J CJ
mouseWB

Int J Biol Sci

Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice

Authors - Min Hu
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca
humanWB

Signal Transduct Target Ther

RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.

Authors - Yalan Lu
humanWB

Metabolites

Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response

Authors - Yun Wu