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TRBV5-4 Polyclonal antibody

TRBV5-4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13524-1-AP

Synonyms

TCRBV5S4, TCRBV5S6A3N2T, TRBV5 4, TRBV54, TRBV5-4

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using TRBV5-4 Polyclonal antibody (13524-1-AP)

    WB analysis of U-937 using 13524-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using TRBV5-4 Polyclonal antibody (13524-1-AP)

    WB analysis of U-937 using 13524-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13524-1-AP targets TRBV5-4 in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRBV5-4 fusion protein Ag4337
Full Name T cell receptor beta variable 5-4
Calculated Molecular Weight 308 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC028083
Gene Symbol TRBV5-4
Gene ID (NCBI) 28611
RRIDAB_1167555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRBV5-4 antibody 13524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols