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RFP2 Polyclonal antibody

RFP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22021-1-AP

Synonyms

CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RFP2 Polyclonal antibody (22021-1-AP)

    WB analysis of Jurkat using 22021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22021-1-AP (RFP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using RFP2 Polyclonal antibody (22021-1-AP)

    IP experiment of Jurkat using 22021-1-AP

    IP result of anti-RFP2 (IP:22021-1-AP, 4ug; Detection:22021-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RFP2 Polyclonal antibody (22021-1-AP)

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RFP2 Polyclonal antibody (22021-1-AP)

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 22021-1-AP is RFP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFP2 fusion protein Ag16805
Full Name tripartite motif-containing 13
Calculated Molecular Weight 407 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC003579
Gene Symbol TRIM13
Gene ID (NCBI) 10206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Publications

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article