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KD/KO Validated

TRIM46 Polyclonal antibody

Name: TRIM46 Polyclonal antibody
Brand: Proteintech
SKU: 21026-1-AP

TRIM46 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21026-1-AP

Synonyms

Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

Validation Data Gallery

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AllWBIHCIF

WB analysis of mouse lung using 21026-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 21026-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21026-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung using 21026-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 21026-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 21026-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21026-1-AP
Immunofluorescent analysis of HepG2 cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 21026-1-AP
Immunofluorescent analysis of Hela cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 21026-1-AP

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Tested Applications

Positive IHC detected in	human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, Hela cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

21026-1-AP targets TRIM46 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM46 fusion protein Ag15290
Full Name	tripartite motif-containing 46
Calculated Molecular Weight	759 aa, 83 kDa
Observed Molecular Weight	55 kDa, 62 kDa
GenBank Accession Number	BC069416
Gene Symbol	TRIM46
Gene ID (NCBI)	80128
RRID	AB_10732843
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM46 antibody 21026-1-AP	Download protocol
IHC protocol for TRIM46 antibody 21026-1-AP	Download protocol
IF protocol for TRIM46 antibody 21026-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation. Authors - John K Vuong View Article
mouse	WB,IF	Cell Rep The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling. Authors - Sotaro Ichinose View Article
human	IHC	Oncol Res Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR. Authors - Wenwei Jiang View Article
human	WB	Exp Cell Res TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination. Authors - Jingfa Zhang KD Validated View Article
human	WB,IF	Int J Mol Sci Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46. Authors - Li Zhang View Article
human	WB,CoIP	Eye Vis (Lond) TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination Authors - Hangqi Shen KO Validated View Article

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Research Area

TRIM46 Polyclonal antibody

TRIM46 Polyclonal Antibody for IF, IHC, WB, ELISA

Validation Data Gallery

WB analysis of mouse lung using 21026-1-AP

WB analysis of mouse testis using 21026-1-AP

WB analysis of HeLa using 21026-1-AP

IHC staining of human lung using 21026-1-AP

IHC staining of human lung using 21026-1-AP

IHC staining of human lung using 21026-1-AP

IF Staining of HepG2 using 21026-1-AP

IF Staining of HeLa using 21026-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.

The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.

Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.

TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.

Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46.

TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination