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  • Featured Product
  • KD/KO Validated

TRIM7 Polyclonal antibody

TRIM7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26285-1-AP

Synonyms

Glycogenin interacting protein, GNIP, RING finger protein 90, RNF90, TRIM7, tripartite motif containing 7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using TRIM7 Polyclonal antibody (26285-1-AP)

    WB analysis of mouse heart using 26285-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26285-1-AP (TRIM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TRIM7 Polyclonal antibody (26285-1-AP)

    IHC staining of mouse skeletal muscle using 26285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26285-1-AP (TRIM7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TRIM7 Polyclonal antibody (26285-1-AP)

    IHC staining of mouse skeletal muscle using 26285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26285-1-AP (TRIM7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26285-1-AP targets TRIM7 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM7 fusion protein Ag23453
Full Name tripartite motif-containing 7
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC132863
Gene Symbol TRIM7
Gene ID (NCBI) 81786
RRIDAB_2880462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM7 antibody 26285-1-APDownload protocol
IHC protocol for TRIM7 antibody 26285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1.

Authors - Chenliang Zhou
View Article
humanWB

J Virol

Nascent Transcriptomics Reveal Cellular Prolytic Factors Upregulated Upstream of the Latent-to-Lytic Switch Protein of Epstein-Barr Virus.

Authors - Tiffany R Frey
  • KD Validated
View Article
humanWB

Vet Microbiol

TRIM7 inhibits encephalomyocarditis virus replication by activating interferon-β signaling pathway

Authors - Minjing Li
View Article