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TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5D8C7

Cat no : 60205-1-Ig

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRIP6 Monoclonal antibody (60205-1-Ig)

    WB analysis of HeLa using 60205-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60205-1-Ig (TRIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human gliomas using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human ovary tumor using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human ovary tumor using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human colon cancer using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human colon cancer using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human colon using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human colon using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human liver using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIP6 Monoclonal antibody (60205-1-Ig)

    IHC staining of human liver using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using TRIP6 Monoclonal antibody (60205-1-Ig)

    FC experiment of HeLa using 60205-1-Ig

    1X10^6 HeLa cells were stained with 0.2ug TRIP6 antibody (60205-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHela cells
Positive IHC detected inhuman ovary tumor tissue, human liver tissue, human colon cancer tissue, human colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60205-1-Ig targets TRIP6 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIP6 fusion protein Ag15149
Full Name thyroid hormone receptor interactor 6
Calculated Molecular Weight 476 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC004249
Gene Symbol TRIP6
Gene ID (NCBI) 7205
RRIDAB_10973674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

Protocols

Product Specific Protocols
WB protocol for TRIP6 antibody 60205-1-IgDownload protocol
IHC protocol for TRIP6 antibody 60205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Dev Cell

Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex.

Authors - Marcela Raices
View Article