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TRMT2B Polyclonal antibody

TRMT2B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20767-1-AP

Synonyms

CXorf34, TRM2 homolog, TRMT2B

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TRMT2B Polyclonal antibody (20767-1-AP)

    WB analysis of HepG2 using 20767-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20767-1-AP (TRMT2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TRMT2B Polyclonal antibody (20767-1-AP)

    WB analysis of HepG2 using 20767-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20767-1-AP (TRMT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRMT2B Polyclonal antibody (20767-1-AP)

    IHC staining of human liver cancer using 20767-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20767-1-AP (TRMT2B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRMT2B Polyclonal antibody (20767-1-AP)

    IHC staining of human liver cancer using 20767-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20767-1-AP (TRMT2B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TRMT2B Polyclonal antibody (20767-1-AP)

    IHC staining of human colon using 20767-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20767-1-AP (TRMT2B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TRMT2B Polyclonal antibody (20767-1-AP)

    IHC staining of human colon using 20767-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20767-1-AP (TRMT2B antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20767-1-AP is TRMT2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT2B fusion protein Ag14750
Full Name TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae)
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC007526
Gene Symbol TRMT2B
Gene ID (NCBI) 79979
RRIDAB_10693626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRMT2B antibody 20767-1-APDownload protocol
IHC protocol for TRMT2B antibody 20767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols