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TROVE2 Polyclonal antibody

TROVE2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12718-1-AP

Synonyms

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of MCF-7 using 12718-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of HeLa using 12718-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of PC-3 using 12718-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of rat spleen using 12718-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of mouse thymus using 12718-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    IF Staining of PC-3 using 12718-1-AP

    Immunofluorescent analysis of PC-3 cells using 12718-1-AP (TROVE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, mouse thymus tissue, PC-3 cells, rat spleen tissue
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12718-1-AP targets TROVE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TROVE2 fusion protein Ag3412
Full Name TROVE domain family, member 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC036658
Gene Symbol TROVE2
Gene ID (NCBI) 6738
RRIDAB_2209756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

Protocols

Product Specific Protocols
WB protocol for TROVE2 antibody 12718-1-APDownload protocol
IF protocol for TROVE2 antibody 12718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.

Authors - Chuang Li
View Article