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TROVE2 Monoclonal antibody

TROVE2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

2A4E5

Cat no : 67149-1-Ig

Synonyms

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of HeLa using 67149-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of HEK-293 using 67149-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of MCF-7 using 67149-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TROVE2 Monoclonal antibody (67149-1-Ig)

    WB analysis of Jurkat using 67149-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TROVE2 Monoclonal antibody (67149-1-Ig)

    IHC staining of mouse brain using 67149-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, HEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67149-1-Ig targets TROVE2 in WB, IHC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TROVE2 fusion protein Ag4379
Full Name TROVE domain family, member 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC036658
Gene Symbol TROVE2
Gene ID (NCBI) 6738
RRIDAB_2882447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

Protocols

Product Specific Protocols
WB protocol for TROVE2 antibody 67149-1-IgDownload protocol
IHC protocol for TROVE2 antibody 67149-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols