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  • Featured Product
  • KD/KO Validated

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 21938-1-AP

Synonyms

GC79, LGCR, TRPS1, Zinc finger protein GC79

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPS1 Polyclonal antibody (21938-1-AP)

    WB analysis using 21938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRPS1 Polyclonal antibody (21938-1-AP)

    WB analysis of HEK-293 using 21938-1-AP

    WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using TRPS1 Polyclonal antibody (21938-1-AP)

    WB analysis of HeLa using 21938-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRPS1 Polyclonal antibody (21938-1-AP)

    WB analysis of HEK-293 using 21938-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using TRPS1 Polyclonal antibody (21938-1-AP)

    IP experiment of mouse lung using 21938-1-AP

    IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using TRPS1 Polyclonal antibody (21938-1-AP)

    IF Staining of mouse skin using 21938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using TRPS1 Polyclonal antibody (21938-1-AP)

    IF Staining of mouse skin using 21938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse testis tissue
Positive IP detected inmouse lung tissue
Positive IF detected inmouse skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21938-1-AP targets TRPS1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPS1 fusion protein Ag16583
Full Name trichorhinophalangeal syndrome I
Calculated Molecular Weight 1294 aa, 143 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC125020
Gene Symbol TRPS1
Gene ID (NCBI) 7227
RRIDAB_11232233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Protocols

Product Specific Protocols
WB protocol for TRPS1 antibody 21938-1-APDownload protocol
IF protocol for TRPS1 antibody 21938-1-APDownload protocol
IP protocol for TRPS1 antibody 21938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols