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- KD/KO Validated
TSP50 Polyclonal antibody
TSP50 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12574-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
Product Information
12574-1-AP targets TSP50 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TSP50 fusion protein Ag3247 |
| Full Name | testes-specific protease 50 |
| Calculated Molecular Weight | 385 aa, 43 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC033016 |
| Gene Symbol | TSP50 |
| Gene ID (NCBI) | 29122 |
| RRID | AB_10646475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSP50 antibody 12574-1-AP | Download protocol |
| IHC protocol for TSP50 antibody 12574-1-AP | Download protocol |
| IF protocol for TSP50 antibody 12574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Cancer Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.
| ||
Oncol Rep Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest. | ||
Gastroenterol Res Pract Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer. | ||
Oncol Lett Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer. |
