TSPY3 Polyclonal antibody

TSPY3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22257-1-AP

Synonyms

Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3



Tested Applications

Positive WB detected inhuman testis tissue, LNCaP cells
Positive IHC detected inhuman testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22257-1-AP targets TSPY3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPY3 fusion protein Ag17762
Full Name testis specific protein, Y-linked 3
Calculated Molecular Weight 308 aa, 35 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC075016
Gene Symbol TSPY3
Gene ID (NCBI) 728137
RRIDAB_11182707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TSPY3 antibody 22257-1-APDownload protocol
IHC protocol for TSPY3 antibody 22257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols