• Featured Product
  • KD/KO Validated

TTBK2 Polyclonal antibody

TTBK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15072-1-AP

Synonyms

KIAA0847, SCA11, tau tubulin kinase 2, TTBK, TTBK2



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15072-1-AP targets TTBK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTBK2 fusion protein Ag7108
Full Name tau tubulin kinase 2
Calculated Molecular Weight 137 kDa
Observed Molecular Weight 137-150 kDa
GenBank Accession NumberBC071556
Gene Symbol TTBK2
Gene ID (NCBI) 146057
RRIDAB_2211507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.

Protocols

Product Specific Protocols
WB protocol for TTBK2 antibody 15072-1-APDownload protocol
IHC protocol for TTBK2 antibody 15072-1-APDownload protocol
IF protocol for TTBK2 antibody 15072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
humanWB

PLoS Genet

The Tau Tubulin Kinases TTBK1/2 Promote Accumulation of Pathological TDP-43.

Authors - Nicole F Liachko
humanIF

Sci Rep

MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.

Authors - Si-Hyung Lee
humanIF

Hum Mol Genet

Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.

Authors - Mairéad Flynn
humanIF

J Cell Sci

Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.

Authors - Ebtissal M Khouj
mouseIF

PLoS Genet

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Authors - Emily Bowie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (01-27-2023)

Used on lysates from HEK293T cells. Incubation for a few hours at room temperature. Detection of 2 main bands, 1 above 118 kDa marker (expected size of TTBK2 is 137-150 kDa) and 1 between 47 and 85 kDa markers.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T