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TTC12 Polyclonal antibody

TTC12 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28216-1-AP

Synonyms

FLJ13859, FLJ20535, TPARM, TPR repeat protein 12, TTC12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC12 Polyclonal antibody (28216-1-AP)

    WB analysis using 28216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28216-1-AP (TTC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTC12 Polyclonal antibody (28216-1-AP)

    IHC staining of human lung cancer using 28216-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTC12 Polyclonal antibody (28216-1-AP)

    IHC staining of human lung cancer using 28216-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TTC12 Polyclonal antibody (28216-1-AP)

    IF Staining of A549 using 28216-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 28216-1-AP (TTC12 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, U-251 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28216-1-AP targets TTC12 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC12 fusion protein Ag28199
Full Name tetratricopeptide repeat domain 12
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC032355
Gene Symbol TTC12
Gene ID (NCBI) 54970
RRIDAB_2881092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC12 antibody 28216-1-APDownload protocol
IHC protocol for TTC12 antibody 28216-1-APDownload protocol
IF protocol for TTC12 antibody 28216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols