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  • Featured Product
  • KD/KO Validated

TTC30A Polyclonal antibody

TTC30A Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25352-1-AP

Synonyms

IFT70, TPR repeat protein 30A, TTC30A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC30A Polyclonal antibody (25352-1-AP)

    WB analysis using 25352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25352-1-AP (TTC30A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TTC30A Polyclonal antibody (25352-1-AP)

    WB analysis of A431 using 25352-1-AP

    WB result of TTC30A antibody (25352-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC30A transfected A431 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TTC30A Polyclonal antibody (25352-1-AP)

    IP experiment of HEK-293 using 25352-1-AP

    IP result of anti-TTC30A (IP:25352-1-AP, 4ug; Detection:25352-1-AP 1:500) with HEK-293 cells lysate 2600ug.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC30A Polyclonal antibody (25352-1-AP)

    IF Staining of hTERT-RPE1 using 25352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (25352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, mouse testis, rat testis
Positive IP detected inHEK-293 cells
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25352-1-AP targets TTC30A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC30A fusion protein Ag18361
Full Name tetratricopeptide repeat domain 30A
Calculated Molecular Weight 665 aa, 76 kDa
Observed Molecular Weight 72-76 kDa
GenBank Accession NumberBC042848
Gene Symbol TTC30A
Gene ID (NCBI) 92104
RRIDAB_2651172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC30A antibody 25352-1-APDownload protocol
IF protocol for TTC30A antibody 25352-1-APDownload protocol
IP protocol for TTC30A antibody 25352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article
humanWB,IF

Biol Open

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Authors - Ryota Takei
View Article