TTC37 Polyclonal antibody

TTC37 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 24594-1-AP

Synonyms

KIAA0372, TPR repeat protein 37, TTC37



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 24594-1-AP is TTC37 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC37 fusion protein Ag18442
Full Name tetratricopeptide repeat domain 37
Calculated Molecular Weight 1564 aa, 175 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC056893
Gene Symbol TTC37
Gene ID (NCBI) 9652
RRIDAB_2879628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Protocols

Product Specific Protocols
WB protocol for TTC37 antibody 24594-1-APDownload protocol
IHC protocol for TTC37 antibody 24594-1-APDownload protocol
IF protocol for TTC37 antibody 24594-1-APDownload protocol
IP protocol for TTC37 antibody 24594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Clin Invest

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Authors - Kun Yang
humanWB

PLoS Genet

A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.

Authors - Masakazu Kohda